Volume 35 Issue 6
Nov.  2018
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XING Jingjing, MA Li'an, YU Weichu. Screening, Determination and Study on the Degradation Property of a Sulfonated Asphalt Degrading Bacteria[J]. DRILLING FLUID & COMPLETION FLUID, 2018, 35(6): 42-48. doi: 10.3969/j.issn.1001-5620.2018.06.008
Citation: XING Jingjing, MA Li'an, YU Weichu. Screening, Determination and Study on the Degradation Property of a Sulfonated Asphalt Degrading Bacteria[J]. DRILLING FLUID & COMPLETION FLUID, 2018, 35(6): 42-48. doi: 10.3969/j.issn.1001-5620.2018.06.008
shu

Screening, Determination and Study on the Degradation Property of a Sulfonated Asphalt Degrading Bacteria

doi: 10.3969/j.issn.1001-5620.2018.06.008

  • 1. State Key Laboratory of Petroleum Pollution Control, Beijing, 102206;

  • 2. School of Life Science, Yangtze University, Jingzhou, Hubei 434025;

  • 3. School of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei 434025

  • Received Date: 2018-09-30
  • Publish Date: 2018-11-30
    Abstract
  • To find out a microbial strain for effective degradation of sulfonate drilling fluid, experiments were conducted using drilling fluid sludge from Kelamayi Oilfield as the source of microbe and sulfonated asphalt as the sole carbon source for concentration, acclimation, isolation and purification. The species of the microbe was determined with physiological biochemistry technology and molecular biotechnology. The degradation behavior of the microbe was studied through single-factor experiment and orthogonal experiment. A microbial strain that is able to effectively and efficiently degrade sulfonated asphalt was found through these experiments, and was named X2, which is an Oceanobacillus (in ocean sediments) determined with physiological biochemistry and molecular characterization. Single-factor experiment showed that the best P-source for X2 to grow is KH2PO4, and the best N-source is NH4NO3. Orthogonal experiment showed that the optimum growing conditions for X2 are as follows:36℃, pH 7.8, 1% salt and inoculum concentration of 10%. The optimum degradation conditions for X2 are as follows:36℃, pH 8.2, 0.9% salt and inoculum concentration of 12%. The rate of degradation at these conditions is 59%-71%. The Range of the pH values is highest and is thus a key factor affecting both the growth of the strain and the COD for X2 to degrade. The experimental results have provided an effective theoretical basis for degrading waste drilling fluids.

     

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