一株磺化沥青降解菌的筛选鉴定及降解特性研究

幸晶晶; 马立安; 余维初

doi:10.3969/j.issn.1001-5620.2018.06.008

一株磺化沥青降解菌的筛选鉴定及降解特性研究

doi: 10.3969/j.issn.1001-5620.2018.06.008

幸晶晶^1,2,
马立安²,
余维初^1,3, ,

1. 石油石化污染物控制与处理国家重点实验室, 北京 102206;
2. 长江大学生命科学学院, 湖北荆州 434025;
3. 长江大学化学与环境工程学院, 湖北荆州 434025

基金项目:

石油石化污染物控制与处理国家重点实验室开放课题资助（PPC2016004）

详细信息

作者简介:
幸晶晶,在读硕士研究生,研究方向为环境微生物学。电话15629866710;E-mail:929674536@qq.com

通讯作者:
余维初,电话13907216790

中图分类号: TE254.4;TE92
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出版历程
- 收稿日期: 2018-09-30
- 刊出日期: 2018-11-30

Screening, Determination and Study on the Degradation Property of a Sulfonated Asphalt Degrading Bacteria

XING Jingjing^1,2,
MA Li'an²,
YU Weichu^{1,3
, ,}

1. State Key Laboratory of Petroleum Pollution Control, Beijing, 102206;
2. School of Life Science, Yangtze University, Jingzhou, Hubei 434025;
3. School of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei 434025

摘要

摘要: 为了选育降解磺化钻井液的微生物菌株，以新疆克拉玛依油田磺化钻井液污泥为微生物菌源，磺化沥青为唯一碳源，进行富集、驯化、分离纯化等筛选有效降解磺化沥青的菌株，经生理生化及分子生物技术鉴定种名，单因素实验和正交实验研究降解特性。结果获得一株有效降解磺化沥青的菌株，命名为X2，生理生化与分子鉴定为大洋沉积物芽孢杆菌。单因素实验表明，X2生长最佳的P源为KH₂PO₄，N源为NH₄NO₃；正交实验结果显示，X2菌体最佳生长条件为温度为36℃、pH值为7.8、盐浓度为1%、接种量为10%；COD降解最佳条件为36℃、pH值为8.2、盐浓度为0.9%、接种量为12%，降解率为59%~71%；pH值的R极差最大，是同时影响菌株生长及COD降解的关键因子。实验结果为降解钻井废液COD提供了有效的理论基础。
- 磺化沥青降解菌 /
- 富集驯化 /
- 筛选鉴定
Abstract: To find out a microbial strain for effective degradation of sulfonate drilling fluid, experiments were conducted using drilling fluid sludge from Kelamayi Oilfield as the source of microbe and sulfonated asphalt as the sole carbon source for concentration, acclimation, isolation and purification. The species of the microbe was determined with physiological biochemistry technology and molecular biotechnology. The degradation behavior of the microbe was studied through single-factor experiment and orthogonal experiment. A microbial strain that is able to effectively and efficiently degrade sulfonated asphalt was found through these experiments, and was named X2, which is an Oceanobacillus (in ocean sediments) determined with physiological biochemistry and molecular characterization. Single-factor experiment showed that the best P-source for X2 to grow is KH₂PO₄, and the best N-source is NH₄NO₃. Orthogonal experiment showed that the optimum growing conditions for X2 are as follows:36℃, pH 7.8, 1% salt and inoculum concentration of 10%. The optimum degradation conditions for X2 are as follows:36℃, pH 8.2, 0.9% salt and inoculum concentration of 12%. The rate of degradation at these conditions is 59%-71%. The Range of the pH values is highest and is thus a key factor affecting both the growth of the strain and the COD for X2 to degrade. The experimental results have provided an effective theoretical basis for degrading waste drilling fluids.
- Sulfonated asphalt degrading bacterium /
- Concentration and acclimation /
- Screening and determination

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